SCI09 7100 Enzymology (เอนไซม์วิทยา)

Properties and control of enzyme activity, enzyme nomenclature and active-site characteristics, thermodynamics of enzyme-catalyzed reactions, orders of reactions and kinetics of Michaelis-Menten, experimental measurements and significance of the kinetic parameters, kinetic of substrate inhibition, kinetics of reversible reactions, and the Haldane relationship, kinetics of competitive, non-competitive, uncompetitive and mixed-type inhibition, effects of pH and temperature on enzyme activity, dose-response curves, protein-ligand binding equilibria, derivation of the Langmuir binding isotherm, binding kinetics using microcalorimetric assay, mechanisms of multisubstrate reactions as described by King-Altman schematic approach, kinetics of allosteric enzymes, enzyme mechanisms and active site determination, practical approaches for determination of enzyme activity.