109703 Protein Structure and Engineering 4(4-0-8)
Prerequisite : 109700 Graduate Biochemistry /Consent of the School
Basic review of the structures and properties of amino acid peptide bonds, peptides, polypeptides & proteins, concepts for protein engineering, protein detection, purification and evaluation, electrophoresis, size determination, quaternary structure evaluation, protein primary structure determination, electronic structures and properties of amino acid residues and their reactivities, chemical synthesis of peptides & proteins; biological nucleic acid and protein synthesis & posttranslational modifications, Methods in genetic engineering of proteins, molecular evolution and the use of protein sequence alignment & analysis, physical basis of size and shape determination (e.g. diffusion, analytical centrifugation, light scattering, small angle X-ray scattering), spectroscopic evaluation of secondary structure and tertiary structure, protein structure determination by X-ray crystallography, NMR, & EM tomography, structural modeling and prediction, protein folding, protein interactions with ligands, proteins in membranes, student proposal on protein engineering.
Expected Learning Outcomes
On completion of this course, students are able to
- explain principles of protein structure and the physical interactions involved in attaining protein structure
- understand the basics of the methods and experiments used to study proten structure and structure-function relationships
- evaluate and analyze scientific papers on protein structure and engineering and evaluate the quality of the data
- have a basic understanding of the methods used to engineer proteins and to evaluate the effects of such engineering
- have conscientious in their academic and professional conduct, and able to work with others
- communicate scientific knowledge appropriately and effectively
- Students are eager for knowledge and learning
